Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus.
Zhou X, Zhong Y, Molinar-Inglis O, Kunkel MT, Chen M, Sun T, Zhang J, Shyy JY, Trejo J, Newton AC, Zhang J
Nat Commun. 2020 Nov 30;11(1):6088. doi: 10.1038/s41467-020-19937-w. PubMed Article
Zhou X, Zhong Y, Molinar-Inglis O, Kunkel MT, Chen M, Sun T, Zhang J, Shyy JY, Trejo J, Newton AC, Zhang J
Nat Commun. 2020 Nov 30;11(1):6088. doi: 10.1038/s41467-020-19937-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175006 | pcDNA3-Akt-STOPS | Akt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity. |
| 175007 | pcDNA3-Akt-STOPS-NLS | Nuclear-targeted Akt-STOPS. |
| 175008 | pcDNA3-Akt-STOPS-NES | Cytosol-targeted Akt-STOPS. |
| 175009 | pcDNA3-Lyn-Akt-STOPS | Plasma membrane-targeted Akt-STOPS. |
| 175010 | pcDNA3-H2A-Akt-STOPS | Akt-STOPS targeted to the nucleus via histone H2A. |
| 175011 | pcDNA3-AktAR2-NES | Cytosol-targeted AktAR2. |
| 175012 | pcDNA3-AktAR2-NLS | Nuclear-targeted AktAR2. |
| 175013 | pcDNA3-Lyn-AktAR2 | Plasma membrane-targeted AktAR2. |
| 175014 | pcDNA3-Akt-Scramble | Negative-control construct for Akt-STOPS. |
| 175015 | pcDNA3-TORCAR-NES | Cytosol-targeted TORCAR. |
| 175016 | pcDNA3-H2A-TORCAR | TORCAR targeted to the nucleus via histone H2A. |