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Structural basis of K63-ubiquitin chain formation by the Gordon-Holmes syndrome RBR E3 ubiquitin ligase RNF216.
Cotton TR, Cobbold SA, Bernardini JP, Richardson LW, Wang XS, Lechtenberg BC
Mol Cell. 2022 Feb 3;82(3):598-615.e8. doi: 10.1016/j.molcel.2021.12.005. Epub 2022 Jan 7. PubMed Article

Plasmids from Article

ID Plasmid Purpose
167354pOPINB-hRNF216Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells
171922pOPINB-hRNF216(457-784)Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.
171923pOPINB-hRNF216(511-784)Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic RBR-helix construct suitable for activity assays.

Antibodies from Article