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Records of RNA locations in living yeast revealed through covalent marks.
Medina-Munoz HC, Lapointe CP, Porter DF, Wickens M
Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23539-23547. doi: 10.1073/pnas.1921408117. Epub 2020 Sep 9. PubMed Article

Plasmids from Article

ID Plasmid Purpose
167452pHCMM1Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter
167453pHCMM2Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter
167454pHCMM3A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]
170359pHCMM4Expresses a Sec61p-mCherry ER-marker from the CYC1 promoter

Antibodies from Article