Endogenous promoter-driven sgRNA for monitoring the expression of low-abundance transcripts and lncRNAs.
Gao N, Hu J, He B, Ji Z, Hu X, Huang J, Wei Y, Peng J, Wei Y, Zhou Y, Shen X, Li H, Feng X, Xiao Q, Shi L, Sun Y, Zhou C, Zhou H, Yang H
Nat Cell Biol. 2021 Jan;23(1):99-108. doi: 10.1038/s41556-020-00610-9. Epub 2021 Jan 4. PubMed Article
Gao N, Hu J, He B, Ji Z, Hu X, Huang J, Wei Y, Peng J, Wei Y, Zhou Y, Shen X, Li H, Feng X, Xiao Q, Shi L, Sun Y, Zhou C, Zhou H, Yang H
Nat Cell Biol. 2021 Jan;23(1):99-108. doi: 10.1038/s41556-020-00610-9. Epub 2021 Jan 4. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
168290 | PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA | CRISPR-activator Suntag-P65-HSF1 (SPH) |
168291 | PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator | optimized miniCMV (OminiCMV) |
168305 | AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA | mediated knock-in of sgRNA precursor |