Myristoylation alone is sufficient for PKA catalytic subunits to associate with the plasma membrane to regulate neuronal functions.
Xiong WH, Qin M, Zhong H
Proc Natl Acad Sci U S A. 2021 Apr 13;118(15). pii: 2021658118. doi: 10.1073/pnas.2021658118. PubMed Article
Xiong WH, Qin M, Zhong H
Proc Natl Acad Sci U S A. 2021 Apr 13;118(15). pii: 2021658118. doi: 10.1073/pnas.2021658118. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 168482 | pCDNA3_PKA-CαN (G2A)-mVenus | Mammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus. |
| 168483 | pCDNA3_PKA-CαN (K8,9A)-mVenus | Mammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus. |
| 168484 | pCDNA3_PKA-CαN (K22,24A)-mVenus | Mammalian expression of the K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus. |
| 168485 | pCDNA3_PKA-CαN (K29,30A)-mVenus | Mammalian expression of the K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus. |
| 168486 | pCDNA3_PKA-CαN15-mVenus | Mammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus. |
| 168487 | pCDNA3_PKA-CαN (K8,9E)-mVenus | Mammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus. |
| 168488 | pCDNA3_PKA-CαN (E12,14K)-mVenus | Mammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus. |
| 168489 | pCDNA3_PKA-RIIα-sREACh | Mammalian expression of mouse PKA regulatory subunit IIα C-terminally tagged by sREACh. |
| 168490 | pCDNA3_PKA-Cα(K8,9A)-mEGFP | Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP. |
| 168491 | pCDNA3_shRNA resistant PKA-Cα(E12,14K)-mEGFP | Mammalian expression of the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP. Silent mutations were introduced to make it resistant to shRNA knock-down. |
| 168492 | pCDNA3_PKA-RIIα | Mammalian expression of mouse PKA regulatory subunit Iiα, untagged. |
| 168493 | pCDNA3_PKA-Cα(K8,9A)-mPAGFP | Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP. |
| 168494 | pCDNA3_PKA-CαN(K8,9A)-mPAGFP | Mammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP. |
| 168495 | pCDNA3_PKA-CαN(E12,14K)-mPAGFP | Mammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP. |
| 168496 | pCDNA3_PKA-CβN15-mVenus | Mammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit β C-terminally tagged by monomeric Venus. |
| 168497 | pCDNA3_dPKA-CAT1N17-mVenus | Mammalian expression of the N-terminal 17 residues of Drosophila PKA catalytic subunit 1 C-terminally tagged by monomeric Venus. |
| 168498 | pCDNA3_PKA-CαN (K8,9R)-mVenus | Mammalian expression of the K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus. |
| 168499 | pCDNA3_PKA-CαN (E12,14A)-mVenus | Mammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus. |