Addgene: Cre/lox-Mediated Chromosomal Integration of Biosynthetic Gene Clusters for Heterologous Expression in Aspergillus nidulans.

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Yit Heng Chooi
Roux et al

Cre/lox-Mediated Chromosomal Integration of Biosynthetic Gene Clusters for Heterologous Expression in Aspergillus nidulans.
Roux I, Chooi YH
ACS Synth Biol. 2022 Feb 16. doi: 10.1021/acssynbio.1c00458. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
168784	pGem-PgpdA-Cre-TtrpC	Helper vector for the transient expression of Cre recombinase in filamentous fungi. Not replicative in fungi.
168785	pGem-LP1-loxP-bar-lox2272-71	Vector to create landing pad 1 (LP1) by homologous recombination in Aspergillus nidulans ΔSt site. The strain A. nidulans LP1 is used for recombinase mediated chromosomal integration.
168786	pGem-LP2-loxP-bar-lox2272-71	Vector to create landing pad 2 (LP2) by homologous recombination in Aspergillus nidulans IS1 site. The strain A. nidulans LP2 is used for recombinase mediated chromosomal integration.
168787	pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66	Donor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.

Antibodies from Article