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Optimized CRISPR tools and site-directed transgenesis towards gene drive development in Culex quinquefasciatus mosquitoes.
Feng X, Lopez Del Amo V, Mameli E, Lee M, Bishop AL, Perrimon N, Gantz VM
Nat Commun. 2021 May 20;12(1):2960. doi: 10.1038/s41467-021-23239-0.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
169238pVMG0146_Cq-U6-1_2xBbsI-gRNAExpression of gRNA in Culex quinquefasciatus or other mosquitoes
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169323pVMG0149_Cq-U6-6_2xBbsI-gRNAExpression of gRNA in Culex quinquefasciatus or other mosquitoes
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169339pVMG0217_Cq-U6-2b_2xBbsI-gRNAExpression of gRNA in Culex quinquefasciatus or other mosquitoes
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169345pVMG0173_Cq-Actin5c-Cas9Expression of Cas9 in Culex quinquefasciatus or other mosquitoes
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169346pVMG0193_Cq-Rpl40-Cas9Expression of Cas9 in Culex quinquefasciatus or other mosquitoes
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169347pVMG0213_Cq-vasa-Cas9Expression of Cas9 in Culex quinquefasciatus or other mosquitoes
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169348pVMG0212_Cq-nanos-Cas9Expression of Cas9 in Culex quinquefasciatus or other mosquitoes
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169369pVMG0302_Cq-U6-1_2xBbsI_gRNA-LoopExpression of gRNA in Culex quinquefasciatus or other mosquitoes
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169370pVMG0303_Cq-U6-6_2xBbsI_gRNA-LoopExpression of gRNA in Culex quinquefasciatus or other mosquitoes
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Antibodies from Article