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An optimized CRISPR/Cas9 approach for precise genome editing in neurons.
Fang H, Bygrave AM, Roth RH, Johnson RC, Huganir RL
Elife. 2021 Mar 10;10. pii: 65202. doi: 10.7554/eLife.65202.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
169416pSGUsed for guide cloning
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169417GluA1 (px330:2-guideCas9)Expression of Cas9 and 2 guides
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169418pMiniT:SEP-GluA1 TKIT donorDonor DNA for TKIT knockin of SEP to GluA1 in mouse
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169419pMiniT:myc-GluA1 TKIT donorDonor DNA for TKIT knockin of Myc to GluA1 in mouse
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169420GluA2 (px330:2-guideCas9)Expression of Cas9 and 2 guides
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169421pMiniT:SEP-GluA2 TKIT donorDonor DNA for TKIT knockin of SEP to GluA2 in mouse
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169423pMiniT:myc-GluA2 TKIT donorDonor DNA for TKIT knockin of Myc to GluA2 in mouse
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169424GluA3 (px330:2-guideCas9)Expression of Cas9 and 2 guides
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169425pMiniT:SEP-GluA3 TKIT donorDonor DNA for TKIT knockin of SEP to GluA3 in mouse
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169426GluN1 (px330:2-guideCas9)Expression of Cas9 and 2 guides
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169427pMiniT:SEP-GluN1 TKIT donorDonor DNA for TKIT knockin of SEP to NR1 in mouse
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169428GluN2A (px330:2-guideCas9)Expression of Cas9 and 2 guides
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169429pMiniT:SEP-GluN2A TKIT donorDonor DNA for TKIT knockin of SEP to NR2A in mouse
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169430GluN2B (px330:2-guideCas9)Expression of Cas9 and 2 guides
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169431pMiniT:SEP-GluN2B TKIT donorDonor DNA for TKIT knockin of SEP to NR2B in mouse
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169432Gephyrin (px330:2-guideCas9)Expression of Cas9 and 2 guides
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169433pMiniT:GFP-Gephyrin TKIT donorDonor DNA for TKIT knockin of GFP to Gephyrin in mouse
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169435beta2 (px330:2-guideCas9)Expression of Cas9 and 2 guides
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169436pMiniT:SEP-beta2 TKIT donorDonor DNA for TKIT knockin of SEP to Gabrb2 in mouse
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169437rGluA1 (px330:2-guideCas9)Expression of Cas9 and 2 guides
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169438pHFS:SEP-rGluA1 TKIT donorDonor DNA for TKIT knockin of SEP to GluA1 in rat
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169439rGluA2 (px330:2-guideCas9)Expression of Cas9 and 2 guides
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169440pHFS:SEP-rGluA2 TKIT donorDonor DNA for TKIT knockin of SEP to GluA2 in rat
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169441pAAV-SEP-GluA1 TKITExpression of 2 guides + donor DNA
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169442pAAV-SEP-GluA2 TKITExpression of 2 guides + donor DNA
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169443pX20Used for guide cloning with PCR
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169444pX52Used for guide cloning with PCR
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Antibodies from Article