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Caffeine-Operated Synthetic Modules for Chemogenetic Control of Protein Activities by Life Style.
Wang T, He L, Jing J, Lan TH, Hong T, Wang F, Huang Y, Ma G, Zhou Y
Adv Sci (Weinh). 2020 Dec 13;8(3):2002148. doi: 10.1002/advs.202002148. eCollection 2021 Feb.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
169683EGFP–acVHH (Y104F)–PBA COSMO mutant (Y104F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
169684EGFP–COSMO–PBCOSMO responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
169685EGFP–acVHH–PBAn anti-caffeine nanobody responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
171071EGFP-acVHH (Y34F)-PBA COSMO mutant (Y34F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
171072EGFP-acVHH (Y34W)-PBA COSMO mutant (Y34W) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

Antibodies from Article