Valence and patterning of aromatic residues determine the phase behavior of prion-like domains.
Martin EW, Holehouse AS, Peran I, Farag M, Incicco JJ, Bremer A, Grace CR, Soranno A, Pappu RV, Mittag T
Science. 2020 Feb 7;367(6478):694-699. doi: 10.1126/science.aaw8653. PubMed Article
Martin EW, Holehouse AS, Peran I, Farag M, Incicco JJ, Bremer A, Grace CR, Soranno A, Pappu RV, Mittag T
Science. 2020 Feb 7;367(6478):694-699. doi: 10.1126/science.aaw8653. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169713 | A1-LCD WT | Bacterial expression of human hnRNPA1-A Low complexity domain including amino acids 186-320 |
| 169714 | A1-LCD deltahexa | Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, hexapeptide including aa 259-264 deleted |
| 169715 | A1-LCD Aro- | Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. |
| 169716 | A1-LCD Aro-- | Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. |
| 169717 | A1-LCD Aro+ | Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. PHE and TYR amino acids shuffled to optimize mixing. |
| 169718 | A1-LCD Perfect Mix | Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. TYR and PHE shuffled into 5 clusters. |
| 169719 | A1-LCD Patchy | Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. |