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Addgene
Allosteric regulation of CAD modulates de novo pyrimidine synthesis during the cell cycle.
Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, Possemato R
Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9. PubMed Article

Plasmids from Article

ID Plasmid Purpose
188117pMXS-IRES-BLAST Halo-flag-CADExpresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells
188118pMXS-IRES-BLAST EGFP-flag-CADExpresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells
188119pMXS-IRES-BLAST flag-CAD S1345AExpresses C-terminal flag-tagged human CAD S1345A in mammalian cells
188120pMXS-IRES-BLAST flag-CAD T1348AExpresses C-terminal flag-tagged human CAD T1348A in mammalian cells
188121pMXS-IRES-BLAST flag-CAD F1366AExpresses C-terminal flag-tagged human CAD F1366A in mammalian cells
188122pMXS-IRES-BLAST flag-CAD G1347AExpresses C-terminal flag-tagged human CAD G1347A in mammalian cells
188123pMXS-IRES-BLAST flag-CAD W1364AExpresses C-terminal flag-tagged human CAD W1364A in mammalian cells
188124pMXS-IRES-BLAST flag-CAD N1394AExpresses C-terminal flag-tagged human CAD N1394A in mammalian cells
188125pMXS-IRES-BLAST flag-CAD L1395AExpresses C-terminal flag-tagged human CAD L1395A in mammalian cells
188126pMXS-IRES-BLAST flag-CAD S1396AExpresses C-terminal flag-tagged human CAD S1396A in mammalian cells
188127pMXS-IRES-BLAST flag-CAD G1412AExpresses C-terminal flag-tagged human CAD G1412A in mammalian cells
188128pMXS-IRES-BLAST flag-CAD G-Loop1Expresses C-terminal flag-tagged human CAD G-Loop1 in mammalian cells
188129pMXS-IRES-BLAST flag-CAD G-Loop2Expresses C-terminal flag-tagged human CAD G-Loop2 in mammalian cells
188130pMXS-IRES-BLAST flag-CAD hCPS1 ADExpresses C-terminal flag-tagged human CAD hCPS1 AD in mammalian cells
188131pMXS-IRES-BLAST flag-CAD R1398AExpresses C-terminal flag-tagged human CAD R1398A in mammalian cells
188132pMXS-IRES-BLAST flag-CAD R1403AExpresses C-terminal flag-tagged human CAD R1403A in mammalian cells
188133pMXS-IRES-BLAST flag-CAD R1404AExpresses C-terminal flag-tagged human CAD R1404A in mammalian cells
188134pMXS-IRES-BLAST flag-CAD R1398A R1403A R1404AExpresses C-terminal flag-tagged human CAD R1398A R1403A R1404A in mammalian cells
188135pMXS-IRES-BLAST flag-CAD R1403A R1404AExpresses C-terminal flag-tagged human CAD R1403A R1404A in mammalian cells
188136pMXS-IRES-BLAST flag-CAD L1405AExpresses C-terminal flag-tagged human CAD L1405A in mammalian cells
188137pMXS-IRES-BLAST flag-CAD A1400EExpresses C-terminal flag-tagged human CAD A1400E in mammalian cells
188138pMXS-IRES-BLAST flag-CAD R1398A A1400E R1403A R1404A L1405AExpresses C-terminal flag-tagged human CAD R1398A A1400E R1403A R1404A L1405A in mammalian cells
188139pMXS-IRES-BLAST flag-CAD C1374SExpresses C-terminal flag-tagged human CAD C1374S in mammalian cells
188140pMXS-IRES-BLAST flag-CAD E1367AExpresses C-terminal flag-tagged human CAD E1367A in mammalian cells
188141pMXS-IRES-BLAST flag-CAD E1368AExpresses C-terminal flag-tagged human CAD E1368A in mammalian cells
188142pMXS-IRES-BLAST flag-CAD E1373AExpresses C-terminal flag-tagged human CAD E1373A in mammalian cells
188143pMXS-IRES-BLAST flag-CAD E1367A E1368AExpresses C-terminal flag-tagged human CAD E1367A E1368A in mammalian cells
188144pMXS-IRES-BLAST flag-CAD E1367A E1368A E1373AExpresses C-terminal flag-tagged human CAD E1367A E1368A E1373A in mammalian cells
188145pMXS-IRES-BLAST flag-CAD D1371AExpresses C-terminal flag-tagged human CAD D1371A in mammalian cells
188146pMXS-IRES-BLAST flag-CAD P1375A P1376AExpresses C-terminal flag-tagged human CAD P1375A P1376A in mammalian cells
188147pMXS-IRES-BLAST flag-CAD 3E-3RExpresses C-terminal flag-tagged human CAD 3E-3R in mammalian cells
188148pMXS-IRES-BLAST flag-CAD 3R-3EExpresses C-terminal flag-tagged human CAD 3R-3E in mammalian cells
188149pMXS-IRES-BLAST flag-CAD 3E@3RExpresses C-terminal flag-tagged human CAD 3E@3R in mammalian cells
188150pMXS-IRES-BLAST flag-CAD hCPS1 TLExpresses C-terminal flag-tagged human CAD hCPS1 TL in mammalian cells

Antibodies from Article