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Enhancing site-specific DNA integration by a Cas9 nuclease fused with a DNA donor-binding domain.
Ma S, Wang X, Hu Y, Lv J, Liu C, Liao K, Guo X, Wang D, Lin Y, Rong Z
Nucleic Acids Res. 2020 Oct 9;48(18):10590-10601. doi: 10.1093/nar/gkaa779.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
169919pCAG-spCas9-SBExpression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.
169920pCAG-spCas9-N57Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.
169921pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTBDNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.

Antibodies from Article