Addgene: Single-component near-infrared optogenetic systems for gene transcription regulation.

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Vladislav Verkhusha
Kaberniuk et al

Single-component near-infrared optogenetic systems for gene transcription regulation.
Kaberniuk AA, Baloban M, Monakhov MV, Shcherbakova DM, Verkhusha VV
Nat Commun. 2021 Jun 23;12(1):3859. doi: 10.1038/s41467-021-24212-7. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
170268	pLEVI(408)-ColE-iLight-msfGFP	Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.
170270	pBAD/His-D-iLight	Expression of iLight with 6xHis N-terminal tag in bacteria.
170271	pG12-SEAP	Secreted alkaline phosphatase (SEAP) expression in mammalian cells. Reporter plasmid.
170272	pG12-Rluc8	Renilla reniformisluciferase (RLuc8) expression in mammlian cells. Reporter plasmid.
170273	pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2	NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.
170274	pAAV-CaMKII-GAL4-iLight-VP16	NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.
170275	pAAV-G12-CheRiff-T2A-mCherry	CheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid.

Antibodies from Article