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Functional visualization of NK Cell-mediated killing of metastatic single tumor cells.
Ichise H, Tsukamoto S, Hirashima T, Konishi Y, Oki C, Tsukiji S, Iwano S, Miyawaki A, Sumiyama K, Terai K, Matsuda M
Elife. 2022 Feb 3;11. pii: 76269. doi: 10.7554/eLife.76269.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
170283pPBbsr2-Necl-5-ScNeoTo monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
170284pT2Aneo-tdTomato-CAAXEncoding membrane-anchored tdTomato.
170290pT2ADW-lox-mCherry-hyBRET-ERK-NLSFor generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.
170293LentiCRISPRv2puro-TyrA knock-out vector for the mouse tyrosinase.
170294LentiCRISPRv2bleo-Necl-5A knock-out vector for the mouse Necl-5
170295LentiCRISPRv2bleo-Nectin-2A knock-out vector for the mouse Nectin-2

Antibodies from Article