Addgene: Computational design of chemogenetic and optogenetic split proteins.

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Klaus Hahn
Dagliyan et al

Computational design of chemogenetic and optogenetic split proteins.
Dagliyan O, Krokhotin A, Ozkan-Dagliyan I, Deiters A, Der CJ, Hahn KM, Dokholyan NV
Nat Commun. 2018 Oct 2;9(1):4042. doi: 10.1038/s41467-018-06531-4. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
170484	SPELL-Lyn Component 1	SPELL Lyn kinase (Constitutively active) N-lobe fused to iFKBP. Split site is 279.
170485	SPELL-Lyn Component 2	SPELL Lyn kinase (Constitutively active) C-lobe fused to FRB. Split site is 279.
170486	SPELL-GDI Component 1	SPELL GDI1 N-lobe fused to iFKBP. Split site is 66.
170487	SPELL-GDI Component 2	SPELL GDI1 C-lobe fused to FRB. Split site is 66
170488	SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1)	SPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1
170489	SPELL-TEV Component 1	TEV (N-lobe) fused with iFKBP
170490	SPELL-TEV Component 2	TEV (C-lobe) fused with FRB
170491	SPELL-ITSN1(DH-PH) Component 1	SPELL ITSN1(DH-PH) component 1. mCherry-ITSN(N-lobe) fused to iFKBP. Split at loop 4 of DH domain.
170492	SPELL-ITSN1(DH-PH) Component 2	SPELL ITSN1(DH-PH) component 2. myc-FRB-ITSN(C-lobe). Split at loop 4 of DH domain.

Antibodies from Article