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A robust method for particulate detection of a genetic tag for 3D electron microscopy.
Rae J, Ferguson C, Ariotti N, Webb RI, Cheng HH, Mead JL, Riches JD, Hunter DJ, Martel N, Baltos J, Christopoulos A, Bryce NS, Cagigas ML, Fonseka S, Sayre ME, Hardeman EC, Gunning PW, Gambin Y, Hall TE, Parton RG
Elife. 2021 Apr 27;10. pii: 64630. doi: 10.7554/eLife.64630.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
170523LifeAct-EGFP-APEX2 in pCSDEST2Vector for expression of LifeAct APEX2 in mammalian cells. Parton lab clone FTE
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170524MmCavin4-APEX2-P2A-mKate2Vector for expression of MmCavin4-APEX2-P2A-mKate2 in mammalian cells. Parton lab clone EJF
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170525SITS-MmCav1-EGFP-APEX2Vector expression of MmCav1-EGFP-APEX2 in Leishmania cell free system. Parton lab clone JEJ
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170526SITS-MmCav1-APEX2Vector expression of MmCav1-APEX2 in Leishmania cell free system. Parton lab clone IYW
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170527pME-MmCav1Entry clone for tagging MmCav1 at the C terminus. Parton lab clone BCJ
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170528pME-MmCavin4Entry clone for tagging MmCavin4 at the C terminus. Parton lab clone ADM
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Recombinant Antibodies from Article