Addgene: SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport.

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Ke Xu
Yan et al

SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport.
Yan R, Chen K, Wang B, Xu K
Dev Cell. 2022 Feb 28;57(4):512-525.e8. doi: 10.1016/j.devcel.2021.12.018. Epub 2022 Jan 19. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
170570	pAPV-DsRed2-ER-5	Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.
170571	pEPV-DsRed2-ER-5	Expresses DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.
170620	pAPV-FLAG-DsRed2-ER-5	Expresses FLAG-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after SP cleavage.
170621	pEPV-FLAG-DsRed2-ER-5	Expresses FLAG-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.
170622	pAPV-DsRed2-FLAG-ER-5	Expresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.
170675	pStr-KDEL_APV-SBP-DsRed2-ER-5	Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.
170676	pStr-KDEL_EPV-SBP-DsRed2-ER-5	Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.
170677	pStr-KDEL_TPV-CXCL9-mCherry-SBP	Expresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal TPV tripeptide (cargo) for RUSH.
170678	pStr-KDEL_EPV-CXCL9-mCherry-SBP	Expresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.
170679	pStr-KDEL_APV-SBP-Dendra2-ER-5	Expresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.
170680	pFLAG-SURF4	Expresses FLAG-tagged SURF4 in mammalian cells.
170681	pAPV-mEmerald-DsRed2-ER-5	Expresses mEmerald-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV and poorly exposed KDEL.
170715	pAPV-DsRed2-mEmerald-ER-5	Expresses DsRed2-mEmerald targeted to the ER lumen. The encoded protein is effectively retrieved back to the ER due to exposed KDEL despite the N-terminal APV.
170717	pmEmerald-ERGIC-53	Expresses mEmerald-tagged human ERGIC-53 at high level in mammalian cells.

Antibodies from Article