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Rapid generation of potent antibodies by autonomous hypermutation in yeast.
Wellner A, McMahon C, Gilman MSA, Clements JR, Clark S, Nguyen KM, Ho MH, Hu VJ, Shin JE, Feldman J, Hauser BM, Caradonna TM, Wingler LM, Schmidt AG, Marks DS, Abraham J, Kruse AC, Liu CC
Nat Chem Biol. 2021 Jun 24. pii: 10.1038/s41589-021-00832-4. doi: 10.1038/s41589-021-00832-4. PubMed Article

Plasmids from Article

ID Plasmid Purpose
170791pAW240P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution
172196pAW212P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

Antibodies from Article