An improved auxin-inducible degron system for fission yeast.
Zhang XR, Zhao L, Suo F, Gao Y, Wu Q, Qi X, Du LL
G3 (Bethesda). 2022 Jan 4;12(1):jkab393. doi: 10.1093/g3journal/jkab393. PubMed Article
Zhang XR, Zhao L, Suo F, Gao Y, Wu Q, Qi X, Du LL
G3 (Bethesda). 2022 Jan 4;12(1):jkab393. doi: 10.1093/g3journal/jkab393. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171124 | pDB4581 | A kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus. |
| 171125 | pDB4697 | A plasmid containing the ura4 genomic region,which flanked by a NotI site at the both ends. It served as the control plasmid or backbone for the ura4 integrated plasmid construction. |
| 171126 | pDB5050 | A plasmid containing the codon-optimized OsTIR1, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system. |
| 171127 | pDB4695 | A plasmid containing the codon-optimized OsTIR1-F74A, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system. |