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TagBiFC technique allows long-term single-molecule tracking of protein-protein interactions in living cells.
Shao S, Zhang H, Zeng Y, Li Y, Sun C, Sun Y
Commun Biol. 2021 Mar 19;4(1):378. doi: 10.1038/s42003-021-01896-7. PubMed Article

Plasmids from Article

ID Plasmid Purpose
171427pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRBExpression of HaloTag N58-(GGS)X6-FRB in mammalian cells
171428pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C58Expression of FKBP-(GGS)X6-HaloTag C58 in mammalian cells
171429pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C261Expression of FKBP-(GGS)X6-HaloTagM C261 in mammalian cells
171431pCDNA3.1(+)-hH2A-(GGS)X6-HaloTag C58Expression of hH2A-(GGS)X6-HaloTag C58 in mammalian cells
171432pCDNA3.1(+)-hH3-(GGS)X6-HaloTag C58Expression of hH3-(GGS)X6-HaloTag C58 in mammalian cells
171434pcDNA3.1(+)-tdMCP2-GyG-(GGS)X6-HaloTag C261Expression of tdMCP2-GyG-(GGS)X6-HaloTag C261 in mammalian cells
171435pcDNA3.1(+)-Lifeact-(GGS)X6-HaloTag C58Expression of Lifeact-(GGS)X6-HaloTag C58 in mammalian cells
171436pcDNA3.1(+)-HaloTag N58-(GGS)X6-LifeactExpression of HaloTag N58-(GGS)X6-Lifeact in mammalian cells
171437pcDNA3.1(+)-c-Fos-(GGS)X6-HaloTag C58Expression of c-Fos-(GGS)X6-HaloTag C58 in mammalian cells
171438pcDNA3.1(+)-HaloTag N58-(GGS)X6-c-JunExpression of HaloTag N58-(GGS)X6-c-Jun in mammalian cells
171785pCDNA3.1(+)-HaloTag N58-(GGS)X6-hH2AExpression of HaloTag N58-(GGS)X6-hH2A in mammalian cells

Antibodies from Article