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Short homology-directed repair using optimized Cas9 in the pathogen Cryptococcus neoformans enables rapid gene deletion and tagging.
Huang MY, Joshi MB, Boucher MJ, Lee S, Loza LC, Gaylord EA, Doering TL, Madhani HD
Genetics. 2021 Oct 23. pii: 6409193. doi: 10.1093/genetics/iyab180. PubMed Article

Plasmids from Article

ID Plasmid Purpose
171686pBHM2329pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR
171687pBHM2403pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.
173441pBHM2404pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template
173442pBHM2406pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

Antibodies from Article