Eliminating base-editor-induced genome-wide and transcriptome-wide off-target mutations.
Wang L, Xue W, Zhang H, Gao R, Qiu H, Wei J, Zhou L, Lei YN, Wu X, Li X, Liu C, Wu J, Chen Q, Ma H, Huang X, Cai C, Zhang Y, Yang B, Yin H, Yang L, Chen J
Nat Cell Biol. 2021 May 10. pii: 10.1038/s41556-021-00671-4. doi: 10.1038/s41556-021-00671-4. PubMed Article
Wang L, Xue W, Zhang H, Gao R, Qiu H, Wei J, Zhou L, Lei YN, Wu X, Li X, Liu C, Wu J, Chen Q, Ma H, Huang X, Cai C, Zhang Y, Yang B, Yin H, Yang L, Chen J
Nat Cell Biol. 2021 May 10. pii: 10.1038/s41556-021-00671-4. doi: 10.1038/s41556-021-00671-4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171691 | pEF1a-nSpCas9 | Expresses nSpCas9 in mammalian cells |
| 171692 | pEF1a-nSpCas9-NG | Expresses nSpCas9-NG in mammalian cells |
| 171693 | pU6-ccdB-boxB-tBE-V5-mA3 | Expresses tBE-V5-mA3 in mammalian cells |
| 171694 | pUC57-sgRNA-MS2-U6 | To construct expression vector for tBE-V5-mA3 |
| 171695 | pU6-SasgRNA scaffold-CMV-nSaCas9 | Expresses nSaCas9 in mammalian cells |