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Simple Affinity-Based Method for Concentrating Viruses from Wastewater Using Engineered Curli Fibers.
Birnbaum DP, Vilardi KJ, Anderson CL, Pinto AJ, Joshi NS
ACS ES T Water. 2022 Nov 11;2(11):1836-1843. doi: 10.1021/acsestwater.1c00208. Epub 2021 Dec 29. PubMed Article

Plasmids from Article

ID Plasmid Purpose
171701pBbB8k-csg-NbMS2Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.
171702pBbB8k-csg-nb112Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.
171703pBbB8k-csg-NbSARSArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA is fused to a cross-reactive nanobody targeting the spike protein of betacoronaviruses via a flexible linker.

Antibodies from Article