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Cell-type-specific, multicolor labeling of endogenous proteins with split fluorescent protein tags in Drosophila.
Kamiyama R, Banzai K, Liu P, Marar A, Tamura R, Jiang F, Fitch MA, Xie J, Kamiyama D
Proc Natl Acad Sci U S A. 2021 Jun 8;118(23). pii: 2024690118. doi: 10.1073/pnas.2024690118.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
172061pACUH-sfcherry2(1-10)UAS vector to express sfcherry2(1-10) in Drosophila
172062pACUH-GFP1-10 secUAS vector to express GFP1-10 in the ER lumen
172063pACUH-GFP1-10 mitochondrial matrixUAS vector to express GFP1-10 in the mitochondrial matrix
172064pBS-KS-attB1-2-PT-SA-SD-0-mTagBFP2(11x7)Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 0 coding intron
172065pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron
172066pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron
172067pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron
172068pBS-KS-attB1-2-PT-SA-SD-1-GFP11Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

Antibodies from Article