dFLASH; dual FLuorescent transcription factor Activity Sensor for Histone integrated live-cell reporting and high-content screening
Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell DL, Peet DJ, Whitelaw ML, Bersten DC
bioRxiv 2023.11.21.568191 Article
Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell DL, Peet DJ, Whitelaw ML, Bersten DC
bioRxiv 2023.11.21.568191 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172326 | pLV-REPORT(EF1a) | LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin |
| 172327 | pLV-REPORT(EF1a)-TTN | LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin |
| 172328 | pLV-REPORT(PGK) | LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A Hygromycin |
| 172330 | pLV-REPORT(PGK/CMV) | LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin |
| 172334 | pLV-REPORT(PGK)-12xHRE | hypoxic responsive reporter |
| 172335 | pLV-REPORT(PGK/CMV)-12xHRE | hypoxic responsive reporter |
| 172336 | pLV-REPORT(PGK/CMV)-GRE | Gal4 Response element |
| 172339 | pLV-REPORT(EF1a no GFP) | mnucTomato |
| 172342 | pLV-REPORT(EF1a no GFP) mnuctdTomato | mnuctdTomato |
| 172352 | pLV-REPORT(EF1a)TetR | Tet Repressor reporter |
| 207169 | pLV-TET2Puro-gal4DBD-miniVPR-HA | Dox Inducible Expression Plasmid for gal4DBD-miniVPR-HA |
| 207171 | pEF-IRES-puro6 gal4DBD-HIFCAD myc tag | expression plasmid for Gal4DBD-HIF-CAD.myc expression |
| 207172 | pEF-IRES-puro6 +gal4DBDHIFCAD pGalO linker | expression plasmid for Gal4DBD-HIF-CAD expression GalO linker |
| 207173 | pLV-tet2puro-gal4DBD-HIFCAD | Dox Inducible Expression Plasmid for gal4DBD-HIF-CAD |
| 207174 | pENTR1a-CRISPRoffv2.1 | Gateway Cloning Vector for CRISPRoffv2.1 |
| 207175 | pLV-EgI-NeoR | Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-NeoR |
| 207176 | pLV-EgI-BlastR | Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-BlastR |
| 207177 | pLV-EgI-HygroR | Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-HygroR |
| 207178 | pLV-EgI-ZeoR | Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-ZeoR |
| 207179 | pLV-SFFVp-gI-BlastR | Gateway compatible Lentiviral Expression Plasmid SFFVp-IRES-BlastR |
| 207180 | pLV-EF1a-CRISPRoffv2.1-IRES-BlastR | Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter |
| 207181 | pLV-SFFVp-CRISPRoffv2.1-IRES-BlastR | Lentiviral Expression Plasmid for CRISPRoff2.1 driven by SFFV Promoter |
| 207182 | pLV-SV40p-gI-BlastR | Gateway compatible Lentiviral Expression Plasmid SV40p-IRES-BlastR |
| 208573 | pLV-REPORT(PGKCMV)-nucTomato-hygro | LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin |
| 208574 | pLV-REPORT(PGK/CMV)-GtwyA | LV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A Hygromycin |
| 208575 | pLV-REPORT (PGK) 12xHRE-NanoLuc | hypoxic response element driven NanoLuc Lentiviral reporter |
| 208576 | pLV-REPORT (PGK) NanoLuc | Empty NanoLuc Lentiviral reporter |