Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Super-resolved 3D tracking of cargo transport through nuclear pore complexes.
Chowdhury R, Sau A, Musser SM
Nat Cell Biol. 2022 Jan;24(1):112-122. doi: 10.1038/s41556-021-00815-6. Epub 2022 Jan 10. PubMed Article

Plasmids from Article

ID Plasmid Purpose
172489pM9-BetaGal-8CPlasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence
172490pET21b-LaG9(S151C)Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).
176151pNLS-2xBFP(4C)Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.

Antibodies from Article