A robust pipeline for rapid production of versatile nanobody repertoires.
Fridy PC, Li Y, Keegan S, Thompson MK, Nudelman I, Scheid JF, Oeffinger M, Nussenzweig MC, Fenyo D, Chait BT, Rout MP
Nat Methods. 2014 Dec;11(12):1253-60. doi: 10.1038/nmeth.3170. Epub 2014 Nov 2. PubMed Article
Fridy PC, Li Y, Keegan S, Thompson MK, Nudelman I, Scheid JF, Oeffinger M, Nussenzweig MC, Fenyo D, Chait BT, Rout MP
Nat Methods. 2014 Dec;11(12):1253-60. doi: 10.1038/nmeth.3170. Epub 2014 Nov 2. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172739 | pET21-pelB-LaG-2 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172740 | pET21-pelB-LaG-3 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172741 | pET21-pelB-LaG-6 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172742 | pET21-pelB-LaG-9 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172743 | pET21-pelB-LaG-10 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172744 | pET21-pelB-LaG-12 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172745 | pET21-pelB-LaG-14 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172746 | pET21-pelB-LaG-16 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172747 | pET21-pelB-LaG-17 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172748 | pET21-pelB-LaG-19 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-19; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172749 | pET21-pelB-LaG-21 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172750 | pET21-pelB-LaG-24 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172751 | pET21-pelB-LaG-26 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172752 | pET21-pelB-LaG-27 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172753 | pET21-pelB-LaG-29 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172754 | pET21-pelB-LaG-30 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172755 | pET21-pelB-LaG-35 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-35; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172756 | pET21-pelB-LaG-37 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172757 | pET21-pelB-LaG-41 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172758 | pET21-pelB-LaG-42 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172759 | pET21-pelB-LaG-43 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-43; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172760 | pET21-pelB-LaG-5 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172761 | pET21-pelB-LaG-8 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172762 | pET21-pelB-LaG-11 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172763 | pET21-pelB-LaG-18 | Bacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site. |
| 172764 | pET21-pelB-LaG-16-4GS-LaG-2 | Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker) |
| 172765 | pET21-pelB-LaG-16-3xFLAG-LaG-2 | Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker) |
| 172766 | pET21-pelB-LaG-41-4GS-LaG-2 | Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker) |
| 172767 | pET21-pelB-LaM-1 | Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag. |
| 172768 | pET21-pelB-LaM-2 | Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag. |
| 172769 | pET21-pelB-LaM-3 | Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag. |
| 172770 | pET21-pelB-LaM-4 | Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag. |
| 172771 | pET21-pelB-LaM-6 | Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag. |
| 172772 | pET21-pelB-LaM-8 | Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag. |