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Auxin-mediated protein depletion for metabolic engineering in terpene-producing yeast.
Lu Z, Peng B, Ebert BE, Dumsday G, Vickers CE
Nat Commun. 2021 Feb 16;12(1):1051. doi: 10.1038/s41467-021-21313-1.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
165046pJAID6AIntegrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae
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165047pJAID5ZIntegrative expression of AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.
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165048pJAID5VYIntegrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.
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165049pJAID6JYIntegrative expression of TRX1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.
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165050pJAID5WYIntegrative expression of SOD1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.
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165051pJAID5XYIntegrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.
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165052pJAID9LIntegrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae
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165053pJAID8ZIntegrative expression of yEGFP and SKP1-fusing rice auxin receptor gene OsTIR1.
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165054pJAID9L104Integrative expression of yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.
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165055pJAID9L105Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.
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165056pJAID8Z104Integrative expression of yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.
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165057pJAID8Z105Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.
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165058pILGFP5V1Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP
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165059pILGFP104Integrative expression of yEGFP-AID*(mini auxin inducible degron)-CUP1
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165060pILGFP105Integrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1
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165061pISKP1-TIR1Integrative expression of SKP1-fusing rice auxin receptor gene OsTIR1
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165062pJT11FIntroducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiae
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165064pILGFP1F5Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator
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165065pILGFP1H5Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator.
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165066pILGFPY2ACharacterisation of 2A-mediated bicistronic expression of Y-FAST and yEGFP
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165067pJT9RFRIntroducing GAL promoters-controlled sequiterpene nerolidol-producing module and Y-FAST and in S. cerevisiae
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165068pIR3DH8Knocking out GAL80 using KlURA3 marker
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165069pIR3DH8KKnocking out GAL80 using KanMX4 marker
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