High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion.
Zhong H, Ceballos CC, Massengill CI, Muniak MA, Ma L, Qin M, Petrie SK, Mao T
Elife. 2021 Jun 8;10. pii: 64911. doi: 10.7554/eLife.64911.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172825 | pX330_ACTB-i1 sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172826 | pX330_ACTB-i2 sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172827 | pX330_ACTB-i3 sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172828 | pX330_ACTB-i4 sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172829 | pX330_ACTB-i5 sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172830 | pX330_ACTB-e1 sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the exon 2 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172831 | pX330_ACTB-e2 sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the exon 2 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172832 | pX330_Actb sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172833 | pX330_rActb sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172834 | pX330_TUBA1B sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172835 | pX330_Tuba1b sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172836 | pX330_TOMM20 sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172837 | pX330_VCL sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172838 | pX330_CALR sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172839 | pX330_Camk2a sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172840 | pX330_rSyn1 sgRNA / hSpCas9 | Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172841 | pX330_DRS-1 sgRNA / hSpCas9 | Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330. |
| 172842 | pUC57-Amp_CRISPIE donor B1 | CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9). |
| 172843 | pUC57-Amp_CRISPIE donor B2 | CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9). |
| 172844 | pUC57-Amp_CRISPIE donor B3 | CRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9). |
| 172845 | pUC57-Amp_CRISPIE donor B4 | CRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9). |
| 172846 | pUC57-Amp_CRISPIE donor B5 | CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9). |
| 172847 | pX330 _HITI donor B6 | DRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9). |
| 172848 | pX330 _CRISPIE donor B7 | DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B7 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by DRS-1 or DRS-2 sgRNA (with SpCas9). |
| 172849 | pX330 _CRISPIE donor B8 | DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B8 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-1 or DRS-2 sgRNA (with SpCas9). |
| 172850 | pX330 _CRISPIE donor B9 | DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B9 (Zhong et al, eLife 2021), mEGFP translational phase (2-2), excised by DRS-1 or DRS-2 sgRNA (with SpCas9). |
| 172851 | pX330 _CRISPIE donor B10 | DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9). |
| 172853 | pAAV_ACTB CRISPIE | Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021. |
| 172854 | pKanCMV_mRuby3 | mRuby3 expressed under the CMV promotor |