pUC57-Amp_CRISPIE donor B3
PurposeCRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||172844||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2671
- Total vector size (bp) 3852
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameCRISPIE designer exon (phase 0-0) encoding mTurquoise2
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUC57-Amp_CRISPIE donor B3 was a gift from Haining Zhong (Addgene plasmid # 172844 ; http://n2t.net/addgene:172844 ; RRID:Addgene_172844)
For your References section:High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion. Zhong H, Ceballos CC, Massengill CI, Muniak MA, Ma L, Qin M, Petrie SK, Mao T. Elife. 2021 Jun 8;10. pii: 64911. doi: 10.7554/eLife.64911. 10.7554/eLife.64911 PubMed 34100715