Overexpression of neural miRNAs miR-9/9* and miR-124 suppresses differentiation to Muller glia and promotes differentiation to neurons in mouse retina in vivo.
Suzuki F, Okuno M, Tanaka T, Sanuki R
Genes Cells. 2020 Nov;25(11):741-752. doi: 10.1111/gtc.12809. Epub 2020 Oct 21. PubMed Article
Suzuki F, Okuno M, Tanaka T, Sanuki R
Genes Cells. 2020 Nov;25(11):741-752. doi: 10.1111/gtc.12809. Epub 2020 Oct 21. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177804 | pCAG-nDsRedFL-intron | An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted. |
| 177805 | pCAG-nDsRedFL-miR9/9* | The pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron. |
| 177806 | pCAG-nDsRedFL-miR124 | The pre-miR-124 sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron. |
| 177807 | pCAG-nDsRedFL-miR9/9*/124 | The pre-miR-9/9* and pre-miR-124 sequences have been inserted into the EcoRV of pCAG-nDsRedFL-intron. |
| 177808 | pCAG-nDsRedFL-miRNT | A nontargeting miRNA based on the pre-miR- 30a structure (Qiu et al., 2008) has been inserted into the EcoRV of pCAG-nDsRedFL-intron. |