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A light-gated transcriptional recorder for detecting cell-cell contacts.

Cho KF, Gillespie SM, Kalogriopoulos NA, Quezada MA, Jacko M, Monje M, Ting AY
Elife. 2022 Mar 21;11:e70881. doi: 10.7554/eLife.70881. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
173113pAAV CMV CCR6-eLOV-TEVcs-Gal4Expresses CCR6 receiver construct in mammalian cells
173114pCAG CAG CCL20-pre-mGRASPExpresses CCL20 sender construct in mammalian cells
173115pCDNA3 CMV myc-Arrestin-TEVpExpresses arrestin receiver construct in mammalian cells
173116pAAV syn CCR6-eLOV-TEVcs-tTAExpresses CCR6 receiver construct in neurons
173117pAAV syn CCL20-pre-mGRASPExpresses CCL20 sender construct in neurons
173118pAAV syn Arrestin-TEVp-V5Expresses arrestin receiver construct in neurons
173119pPB EF1a CCR6-eLOV-TEVcs-Gal4Expresses CCR6 eLOV receiver construct in DIPG
173120pPB EF1a CCR6-hLOV-TEVcs-Gal4Expresses CCR6 hLOV receiver construct in DIPG
173121pPB UbC Arrestin-TEVp(WT)-V5; UAS-mCherryExpresses TEVp(WT) and reporter receiver construct in DIPG
173122pPB UbC Arrestin-uTEV1-V5; UAS-mCherryExpresses uTEV1 and reporter receiver construct in DIPG
173123pPB UbC Arrestin-uTEV2-V5; UAS-mCherryExpresses uTEV2 and reporter receiver construct in DIPG

Antibodies from Article