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CHP1 Regulates Compartmentalized Glycerolipid Synthesis by Activating GPAT4.
Zhu XG, Nicholson Puthenveedu S, Shen Y, La K, Ozlu C, Wang T, Klompstra D, Gultekin Y, Chi J, Fidelin J, Peng T, Molina H, Hang HC, Min W, Birsoy K
Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi: 10.1016/j.molcel.2019.01.037. Epub 2019 Mar 4. PubMed Article

Plasmids from Article

ID Plasmid Purpose
173164pMXS-IRES-Blast ACSL4Retroviral vector to express sgRNA resistant human ACSL4
173165pMXS-IRES-Blast ACSL3Retroviral vector to express sgRNA resistant human ACSL3
173166pMXS-IRES-Blast 3XFLAG-CHP1Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1
173167pMXS-IRES-Blast 3XFLAG-CHP1-myr_mutRetroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutation
173168pMXS-IRES-Blast HA-GPAT4Retroviral vector to express sgRNA resistant HA tagged human GPAT4
173169pMXS-IRES-Blast HA-GPAT4-ΔNs5Retroviral vector to express sgRNA resistant HA tagged human GPAT4 with mutation in CHP1 binding site

Antibodies from Article