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Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs.
Xu P, Liu Z, Liu Y, Ma H, Xu Y, Bao Y, Zhu S, Cao Z, Wu Z, Zhou Z, Wei W
Nat Biotechnol. 2021 Jun 21. pii: 10.1038/s41587-021-00944-1. doi: 10.1038/s41587-021-00944-1.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
174163BARBEKO sgRNA libraryiBARed cytosine Base Editing-mediated gene KnockOut (BARBEKO) sgRNA library; This library leverages CRISPR cytosine base editors for genome-scale knockout screens.
174164pLenti-AncBE4maxThis is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.