Catalytic turnover-based phage selection for engineering the substrate specificity of Sfp phosphopantetheinyl transferase.
Sunbul M, Marshall NJ, Zou Y, Zhang K, Yin J
J Mol Biol. 2009 Apr 10;387(4):883-98. doi: 10.1016/j.jmb.2009.02.010. PubMed Article
Sunbul M, Marshall NJ, Zou Y, Zhang K, Yin J
J Mol Biol. 2009 Apr 10;387(4):883-98. doi: 10.1016/j.jmb.2009.02.010. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 173491 | pET-Sfp R4-4 mutant | Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins. |