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Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling.
Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn MP, Unruh JR, Florens L, Conaway RC, Conaway JW
Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 10.1073/pnas.2107277118.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
173932pET41sIII_GST-ALC1Bacterial expression of ALC1
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173933pET28_6xHis-PARP1delZn1 Bacterial expression of PARP1 lacking Zn1 domain
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173934pET28_6xHis-PARP1delZn3-L713FBacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation
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173935pET28_6xHis-PARP1delZn2Bacterial expression of PARP1 lacking Zn2 domain
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173936pET28_6xHis-PARP1delZn3Bacterial expression of PARP1 lacking Zn3 domain
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173937pET28_6xHis-PARP1delZn1-L713FBacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation
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173938pET28_6xHis-PARP1delBRCTBacterial expression of PARP1 lacking BRCT domain
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173939pET28_6xHis-PARP1delZn2delBRCTBacterial expression of PARP1 lacking Zn2 and BRCT domains
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173940pET28_6xHis-PARP1delWGRBacterial expression of PARP1 lacking WGR domain
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173941pET28_6xHis-PARP1delWGR-L713F Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation
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173942pET28_6xHis-PARP1delCATBacterial expression of PARP1 lacking CAT domain
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173943pET28_6xHis-PARP1-CATBacterial expression of isolated PARP1 CAT domain
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173944pET28_6xHis-PARP1-CAT-L713FBacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation
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173945pET28_6xHis-PARP1-E988KBacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)
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Recombinant Antibodies from Article