Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling.
Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn MP, Unruh JR, Florens L, Conaway RC, Conaway JW
Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 10.1073/pnas.2107277118. PubMed Article
Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn MP, Unruh JR, Florens L, Conaway RC, Conaway JW
Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 10.1073/pnas.2107277118. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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173932 | pET41sIII_GST-ALC1 | Bacterial expression of ALC1 |
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173933 | pET28_6xHis-PARP1delZn1 | Bacterial expression of PARP1 lacking Zn1 domain |
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173934 | pET28_6xHis-PARP1delZn3-L713F | Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation |
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173935 | pET28_6xHis-PARP1delZn2 | Bacterial expression of PARP1 lacking Zn2 domain |
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173936 | pET28_6xHis-PARP1delZn3 | Bacterial expression of PARP1 lacking Zn3 domain |
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173937 | pET28_6xHis-PARP1delZn1-L713F | Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation |
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173938 | pET28_6xHis-PARP1delBRCT | Bacterial expression of PARP1 lacking BRCT domain |
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173939 | pET28_6xHis-PARP1delZn2delBRCT | Bacterial expression of PARP1 lacking Zn2 and BRCT domains |
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173940 | pET28_6xHis-PARP1delWGR | Bacterial expression of PARP1 lacking WGR domain |
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173941 | pET28_6xHis-PARP1delWGR-L713F | Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation |
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173942 | pET28_6xHis-PARP1delCAT | Bacterial expression of PARP1 lacking CAT domain |
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173943 | pET28_6xHis-PARP1-CAT | Bacterial expression of isolated PARP1 CAT domain |
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173944 | pET28_6xHis-PARP1-CAT-L713F | Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation |
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173945 | pET28_6xHis-PARP1-E988K | Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1) |
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