BAd-CRISPR: inducible gene knockout in interscapular brown adipose tissue of adult mice.
Romanelli SM, Lewis KT, Nishii A, Rupp AC, Li Z, Mori H, Schill RL, Learman BS, Rhodes CJ, MacDougald OA
J Biol Chem. 2021 Nov 11:101402. doi: 10.1016/j.jbc.2021.101402. PubMed Article
Romanelli SM, Lewis KT, Nishii A, Rupp AC, Li Z, Mori H, Schill RL, Learman BS, Rhodes CJ, MacDougald OA
J Biol Chem. 2021 Nov 11:101402. doi: 10.1016/j.jbc.2021.101402. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
174540 | AAV sgRNA Expression Plasmid | Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker. |