An integrated pipeline for mammalian genetic screening.
Kramme C, Plesa AM, Wang HH, Wolf B, Smela MP, Guo X, Kohman RE, Chatterjee P, Church GM
Cell Rep Methods. 2021 Sep 27;1(6):100082. doi: 10.1016/j.crmeth.2021.100082. eCollection 2021 Oct 25. PubMed Article
Kramme C, Plesa AM, Wang HH, Wolf B, Smela MP, Guo X, Kohman RE, Chatterjee P, Church GM
Cell Rep Methods. 2021 Sep 27;1(6):100082. doi: 10.1016/j.crmeth.2021.100082. eCollection 2021 Oct 25. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175188 | PB-T3G-ERP2 | All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance) |
| 175267 | pPB-EF1a-MegaGate-DD-Hygro | MegaGate destination with C-terminal DD tag and hygromycin resistance |
| 175268 | pPB-EF1a-MegaGate-HA-Hygro | MegaGate destination with C-terminal HA tag and hygromycin resistance |
| 175270 | pPB-EF1a-MegaGate-HA-Blast | MegaGate destination with C-terminal HA tag and blasticidin resistance |
| 175271 | pPB-EF1a-MegaGate-DD-Blast | MegaGate destination with C-terminal DD tag and blasticidin resistance |
| 175497 | PB-CT3G-ERP2 | All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance) |
| 175498 | PB-T3G-CERP2 | All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance) |
| 175499 | PB-CT3G-CERP2 | All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance) |
| 175501 | PB-CT3G-ERP2-MG | All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance) |
| 175503 | PB-CT3G-CERP2-MG | All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance) |
| 175504 | PB-hEF1a-MG | All-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elements |
| 175505 | PB-hEF1A-MG-U6-sgRNA | All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs |
| 175506 | PB-CT3G-ERP2-MG-IRES2-mNeonGreen | All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance) |
| 175507 | PB-CT3G-CERP2-MG-IRES2-mCherry | All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance) |
| 175508 | PB-CT3G-CERP2-MG-IRES2-mNeonGreen | All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance) |
| 175509 | PB-CT3G-CERP2-MG-IRES2-mTagBFP2 | All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance) |