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ppGpp is a bacterial cell size regulator.
Buke F, Grilli J, Cosentino Lagomarsino M, Bokinsky G, Tans SJ
Curr Biol. 2022 Feb 28;32(4):870-877.e5. doi: 10.1016/j.cub.2021.12.033. Epub 2022 Jan 5. PubMed Article

Plasmids from Article

ID Plasmid Purpose
175590pRelA*Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
175591pMesh1*Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.
175592pYFPTet inducible, KAN Resistant, mVenus gene on a low copy backbone. Used as a negative control to the pRelA_YFP plasmid.
175593pCFPLac inducible, AMP Resistant, cerulean gene on a low copy backbone. Used as a negative control to the pMesh1_CFP plasmid.
175594pMesh1Lac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.
175595pRelA'Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

Antibodies from Article