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Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries.
Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 10.1021/acssynbio.1c00408. Epub 2021 Dec 3. PubMed Article

Plasmids from Article

ID Plasmid Purpose
176152pMC-X2preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-2
176153pMC-X3preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-3
176154pMC-X4preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4
176155pMC-XI1preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-1
176156pMC-XI2preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2
176157pMC-XI3preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3
176158pMC-XI5preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-5
176159pMC-XII1preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1
176160pMC-XII2preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-2
176161pMC-XII5preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-5
176162pMC8b-X2upyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-2
176163pMC7-X2dwyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-2
176164pMC8b-X4upyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-4
176165pMC7-X4dwyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-4
176166pMC8b-XI1upyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-1
176167pMC7-XI1dwyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-1
176168pMC8b-XI2upyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-2
176169pMC7-XI2dwyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-2
176170pMC8b-XI3upyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-3
176171pMC7-XI3dwyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-3
176172pMC8b-XI5upyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-5
176173pMC7-XI5dwyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-5
176174pMC8b-XII2upyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-2
176175pMC7-XII2dwyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-2
176176pMC8b-XII5upyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-5
176177pMC7-XII5dwyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-5
176178pMC6-spaceryeast MoClo level-0 part plasmid type 6 containing non-coding DNA spacer for construction of MoClo plasmids without yeast marker
176179pMC234r-ccdB/TPK2yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)
176180pMC3-TPK2yeast MoClo level-0 part plasmid type 3 containing TPK2 gene
176181pMC3-yeGFPyeast MoClo level-0 part plasmid type 3 containing yeast-enhanced GFP
176182pMC-Ura-Cen-lvl1preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker
176183pMG343preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker
176184pMCL8gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t
176185pMCL8_28bpplasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t
176186pMCL92 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1t

Antibodies from Article