Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Highly Efficient Genome Editing in Plant Protoplasts by Ribonucleoprotein Delivery of CRISPR-Cas12a Nucleases.
Zhang Y, Cheng Y, Fang H, Roberts N, Zhang L, Vakulskas CA, Niedz RP, Culver JN, Qi Y
Front Genome Ed. 2022 Jan 31;4:780238. doi: 10.3389/fgeed.2022.780238. eCollection 2022.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
176889pYPQ220-UltraGateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter
176890pYPQ230-E795LGateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter

Antibodies from Article