Addgene: Reconstitution and use of highly active human CDK1:Cyclin-B:CKS1 complexes.

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Andrea Musacchio
Huis In 't Veld et al

Reconstitution and use of highly active human CDK1:Cyclin-B:CKS1 complexes.
Huis In 't Veld PJ, Wohlgemuth S, Koerner C, Muller F, Janning P, Musacchio A
Protein Sci. 2021 Nov 17. doi: 10.1002/pro.4233. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
177011	pLIB-GST-3C-CDK1	Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)
177012	pLIB-HIS-TEV-Cyclin-B	Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV
177013	pLIB-HIS-TEV-CKS1	Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV
177014	pLIB-HIS-TEV-scCAK1	Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

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