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Quantitative Profiling of WNT-3A Binding to All Human Frizzled Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.
Kozielewicz P, Shekhani R, Moser S, Bowin CF, Wesslowski J, Davidson G, Schulte G
ACS Pharmacol Transl Sci. 2021 May 11;4(3):1235-1245. doi: 10.1021/acsptsci.1c00084. eCollection 2021 Jun 11.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
195839HiBiT-FZD1Expresses human FZD1 with an N-terminal HiBiT
195840HiBiT-FZD2Expresses human FZD2 with an N-terminal HiBiT
195841HiBiT-FZD3Expresses human FZD3 with an N-terminal HiBiT; contains I292V and V495I
195842HiBiT-FZD4Expresses human FZD4 with an N-terminal HiBiT
195843HiBiT-FZD5Expresses human FZD5 with an N-terminal HiBiT
195844HiBiT-FZD6Expresses human FZD6 with an N-terminal HiBiT; contains M345L
195845HiBiT-FZD7Expresses human FZD7 with an N-terminal HiBiT
195846HiBiT-FZD8Expresses human FZD8 with an N-terminal HiBiT
195847HiBiT-FZD9Expresses human FZD9 with an N-terminal HiBiT; contains P199R; I448V; S524N; R556H
195848HiBiT-FZD10Expresses human FZD10 with an N-terminal HiBiT

Antibodies from Article