CRISPR somatic genome engineering and cancer modeling in the mouse pancreas and liver.
Kaltenbacher T, Loprich J, Maresch R, Weber J, Muller S, Oellinger R, Gross N, Griger J, de Andrade Kratzig N, Avramopoulos P, Ramanujam D, Brummer S, Widholz SA, Barthel S, Falcomata C, Pfaus A, Alnatsha A, Mayerle J, Schmidt-Supprian M, Reichert M, Schneider G, Ehmer U, Braun CJ, Saur D, Engelhardt S, Rad R
Nat Protoc. 2022 Apr;17(4):1142-1188. doi: 10.1038/s41596-021-00677-0. Epub 2022 Mar 14. PubMed Article
Kaltenbacher T, Loprich J, Maresch R, Weber J, Muller S, Oellinger R, Gross N, Griger J, de Andrade Kratzig N, Avramopoulos P, Ramanujam D, Brummer S, Widholz SA, Barthel S, Falcomata C, Pfaus A, Alnatsha A, Mayerle J, Schmidt-Supprian M, Reichert M, Schneider G, Ehmer U, Braun CJ, Saur D, Engelhardt S, Rad R
Nat Protoc. 2022 Apr;17(4):1142-1188. doi: 10.1038/s41596-021-00677-0. Epub 2022 Mar 14. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177933 | scAAV-Cre | Expresses Cre-recombinase and can be packaged into AAV particles. |
| 177935 | scAAV-sgRNA-GFP | Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice |
| 177936 | CRISPR-SB | Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase. |