Repurposing an adenine riboswitch into a fluorogenic imaging and sensing tag.
Dey SK, Filonov GS, Olarerin-George AO, Jackson BT, Finley LWS, Jaffrey SR
Nat Chem Biol. 2021 Dec 22. pii: 10.1038/s41589-021-00925-0. doi: 10.1038/s41589-021-00925-0. PubMed Article
Dey SK, Filonov GS, Olarerin-George AO, Jackson BT, Finley LWS, Jaffrey SR
Nat Chem Biol. 2021 Dec 22. pii: 10.1038/s41589-021-00925-0. doi: 10.1038/s41589-021-00925-0. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177913 | pAV-U6+27-Squash | Expresses Squash aptamer in mammalian cells using the U6+27 promoter. |
| 177914 | pAV-5S-Squash | Expresses 5S rRNA fused to Squash aptamer in mammalian cells |
| 177915 | pAV-U6+27-Tornado-Squash | Expresses Squash aptamer as circular RNA in mammalian cells using the Tornado expression system. |
| 177916 | pAV-U6+27-Tornado-Squash-SAM-sensor 4-2 | Expresses Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system. |
| 177917 | pAV-U6+27-Tornado-Squash-SAM-sensor 5-1 | Expresses Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system. |
| 177918 | pAV-U6+27-Tornado-F30-Squash-Broccoli | Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system. |
| 177919 | pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2 | Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system. |
| 177920 | pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 5-1 | Expresses ratiometric Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system. |