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A versatile viral toolkit for functional discovery in the nervous system.
Pouchelon G, Vergara J, McMahon J, Gorissen BL, Lin JD, Vormstein-Schneider D, Niehaus JL, Burbridge TJ, Wester JC, Sherer M, Fernandez-Otero M, Allaway KC, Pelkey K, Chittajallu R, McBain CJ, Fan M, Nasse JS, Wildenberg GA, Fishell G, Dimidschstein J
Cell Rep Methods. 2022 May 26;2(6):100225. doi: 10.1016/j.crmeth.2022.100225. eCollection 2022 Jun 20. PubMed Article

Plasmids from Article

ID Plasmid Purpose
170846pAAV-VTKD1AAV backbone for transgene expression in cortical interneurons under the control of the Dlx enhancer.
170847pAAV-VTKD2AAV backbone for Cre-dependent transgene expression of any transgene in cortical interneurons under the control of the Dlx enhancer.
170848pAAV-VTKD3AAV backbone for Flp-dependent transgene expression of any transgene in cortical interneurons under the control of the Dlx enhancer.
170849pAAV-VTKD4AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of any transgene in cortical interneurons under the control of the Dlx enhancer.
170850pAAV-VTKD5AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of any transgene in cortical interneurons under the control of the Dlx enhancer.
170851pAAV-VTKD6AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in cortical interneurons under the control of the Dlx enhancer.
170852pAAV-VTKS1AAV backbone for transgene expression in neurons under the control of the hSyn1 promoter.
170853pAAV-VTKS2AAV backbone for Cre-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter.
170854pAAV-VTKS3AAV backbone for Flp-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter.
170855pAAV-VTKS4AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.
170856pAAV-VTKS5AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of any transgene in neurons under the control of the hSyn1 promoter.
170857pAAV-VTKS6AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.
178696pAAV-VTKD2-V5-APEX2-NESAAV vector for Cre-dependent transgene expression of V5-APEX-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178697pAAV-VTKD3-V5-APEX2-NESAAV vector for Flp-dependent transgene expression of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178698pAAV-VTKD4-V5-APEX2-NESAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178699pAAV-VTKD5-V5-APEX2-NESAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178700pAAV-VTKD6-V5-APEX2-NESAAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178702pAAV-VTKS2-V5-APEX2-NESAAV vector for Cre-dependent transgene expression of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178703pAAV-VTKS3-V5-APEX2-NESAAV vector for Flp-dependent transgene expression of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178704pAAV-VTKS4-V5-APEX2-NESAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178706pAAV-VTKS1-hChR2-tBFPAAV vector for transgene expression of hChR2-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178707pAAV-VTKS2-Dreadd-hM3Dq-HA-P2A-tBFPAAV vector for Cre-dependent transgene expression of Dreadd-hM3Dq-HA-P2A-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178708pAAV-VTKS3-TeTLc-P2A-mCherryAAV vector for Flp-dependent transgene expression of TeTLc-P2A-mCherry in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178709pAAV-VTKD4-GFPAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178710pAAV-VTKD5-GFPAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178711pAAV-VTKD6-GFPAAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178712pAAV-VTKS4-GFPAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178713pAAV-VTKS5-GFPAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178714pAAV-VTKS6-GFPAAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178715pAAV-VTKD4-dTomAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of dTom in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178716pAAV-VTKD5-dTomAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of dTom in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178717pAAV-VTKD6-dTomAAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of dTom in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178718pAAV-VTKS4-dTomAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178719pAAV-VTKS5-dTomAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178720pAAV-VTKS6-dTomAAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178721pAAV-VTKD4-ChR2-YFPAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178722pAAV-VTKD5-ChR2-YFPAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178723pAAV-VTKD6-ChR2-YFPAAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178724pAAV-VTKS4-ChR2-YFPAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of ChR2-YFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178725pAAV-VTKS5-ChR2-YFPAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of ChR2-YFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178726pAAV-VTKS6-ChR2-YFPAAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of ChR2-YFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178727pAAV-VTKD4-GCaMP6fAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178728pAAV-VTKD5-GCaMP6fAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178729pAAV-VTKD6-GCaMP6fAAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)
178730pAAV-VTKS4-GCaMP6fAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178731pAAV-VTKS5-GCaMP6fAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)
178732pAAV-VTKS6-GCaMP6fAAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Antibodies from Article