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Rapid cell-free characterization of multi-subunit CRISPR effectors and transposons.
Wimmer F, Mougiakos I, Englert F, Beisel CL
Mol Cell. 2022 Feb 16. pii: S1097-2765(22)00102-2. doi: 10.1016/j.molcel.2022.01.026. PubMed Article

Plasmids from Article

ID Plasmid Purpose
178735pAc2_Cas8Expression of A. chroococcum type I-E #2 cas8
178736pAc2_Cse2Expression of A. chroococcum type I-E #2 cse2
178737pAc2_Cas5Expression of A. chroococcum type I-E #2 cas5
178738pAc2_Cas6Expression of A. chroococcum type I-E #2 cas6
178739pAc2_Cas7Expression of A. chroococcum type I-E #2 cas7
178740pAc2_gRNA1Expression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #2 system
178741pAc3_Cas8Expression of A. chroococcum type I-E #3 cas8
178742pAc3_Cse2Expression of A. chroococcum type I-E #3 cse2
178743pAc3_Cas5Expression of A. chroococcum type I-E #3 cas5
178744pAc3_Cas6Expression of A. chroococcum type I-E #3 cas6
178745pAc3_Cas7Expression of A. chroococcum type I-E #3 cas7
178746pAc3_gRNA1Expression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #3 system
178747pLm_Cas8Expression of L. mobilis type I-E cas8
178748pLm_Cse2Expression of L. mobilis type I-E cse2
178749pLm_Cas5Expression of L. mobilis type I-E cas5
178750pLm_Cas6Expression of L. mobilis type I-E cas6
178751pLm_Cas7Expression of L. mobilis type I-E cas7
178752pLm_gRNA1Expression of a gRNA that targets next to PAM library, used for L. mobilis type I-E system
178753pEh_Cas8Expression of E. haloalkaliphila type I-E cas8
178754pEh_Cse2Expression of E. haloalkaliphila type I-E cse2
178755pEh_Cas5Expression of E. haloalkaliphila type I-E cas5
178756pEh_Cas6Expression of E. haloalkaliphila type I-E cas6
178757pEh_Cas7Expression of E. haloalkaliphila type I-E cas7
178758pEh_gRNA1Expression of a gRNA that targets next to PAM library, used for E. haloalkaliphila type I-E system
178759pMs_Cas8Expression of Marinomonas sp. type I-E cas8
178760pMs_Cse2Expression of Marinomonas sp. type I-E cse2
178761pMs_Cas5Expression of Marinomonas sp. type I-E cas5
178762pMs_Cas6Expression of Marinomonas sp. type I-E cas6
178763pMs_Cas7Expression of Marinomonas sp. type I-E cas7
178764pMs_gRNA1Expression of a gRNA that targets next to PAM library, used for Marinomonas sp. type I-E system
178765pXalb_IC_CascadeExpression of X. albilineans type I-C Cascade genes
178766pXalb_IC_Cas3Expression of X. albilineans type I-C cas3
178767Xalb_I-C_array_GGGolden Gate vector to generate X. albilineans type I-C single arrays
178768pXalb_IF1_CascadeExpression of X. albilineansCascade type I-F1 Cascade genes
178769pXalb_IF1_Cas2-3Expression of X. albilineans type I-F1 cas2-3
178770Xalb_I-F1_array_GGGolden Gate vector to generate X. albilineans type I-F1 single arrays
178771pRoCascade_gfpExpression of Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis
178772pRoTnsABCDQExpression of RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis
178773pRoDonorExpression of RoCAST left end, cam, RoCAST right end from Rippkaea orientalis
178774pRoTargetpartial tRNA-Leugene from Rippkaea orientalis
178775Sth_I-E array_GFP dropoutGolden Gate GFP dropout vector to generate S. thermophilus type I-E single arrays
178776pSth_Cas8Expression of S. thermophilus type I-E cas8
178777pSth_Cse2Expression of S. thermophilus type I-E cse2
178778pSth_Cas5Expression of S. thermophilus type I-E cas5
178779pSth_Cas6Expression of S. thermophilus type I-E cas6
178780pSth_Cas7Expression of S. thermophilus type I-E cas7

Antibodies from Article