Base editing sensor libraries for high-throughput engineering and functional analysis of cancer-associated single nucleotide variants.
Sanchez-Rivera FJ, Diaz BJ, Kastenhuber ER, Schmidt H, Katti A, Kennedy M, Tem V, Ho YJ, Leibold J, Paffenholz SV, Barriga FM, Chu K, Goswami S, Wuest AN, Simon JM, Tsanov KM, Chakravarty D, Zhang H, Leslie CS, Lowe SW, Dow LE
Nat Biotechnol. 2022 Feb 14. pii: 10.1038/s41587-021-01172-3. doi: 10.1038/s41587-021-01172-3. PubMed Article
Sanchez-Rivera FJ, Diaz BJ, Kastenhuber ER, Schmidt H, Katti A, Kennedy M, Tem V, Ho YJ, Leibold J, Paffenholz SV, Barriga FM, Chu K, Goswami S, Wuest AN, Simon JM, Tsanov KM, Chakravarty D, Zhang H, Leslie CS, Lowe SW, Dow LE
Nat Biotechnol. 2022 Feb 14. pii: 10.1038/s41587-021-01172-3. doi: 10.1038/s41587-021-01172-3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179217 | Mouse Base Editing Sensor Library | sgRNAs that target frequently occurring cancer associated mutations identified in the MSK IMPACT dataset that can be modeled with cytosine base editing. |
| 179218 | Human Base Editing Sensor Library | sgRNAs that target frequently occurring cancer associated mutations identified in the MSK IMPACT dataset that can be modeled with cytosine base editing. |
| 179219 | ALL-PAM sensor Library | 10 human and mouse sgRNAs each where for each sgRNA the tethered target site has 1 of 64 possible 3 bp PAM sequences. |