Protease-controlled secretion and display of intercellular signals.
Vlahos AE, Kang J, Aldrete CA, Zhu R, Chong LS, Elowitz MB, Gao XJ
Nat Commun. 2022 Feb 17;13(1):912. doi: 10.1038/s41467-022-28623-y. PubMed Article
Vlahos AE, Kang J, Aldrete CA, Zhu R, Chong LS, Elowitz MB, Gao XJ
Nat Commun. 2022 Feb 17;13(1):912. doi: 10.1038/s41467-022-28623-y. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179633 | CMVTO-SEAP-26Sfur-3TM-tevs-AAMP | SEAP RELEASE construct with AAMP motif control |
| 179634 | CMVTO-SEAP-26Sfur-3TM-tevs-KKMP | TEVP-inducible tri-transmembrane SEAP RELEASE construct |
| 179635 | CMVTO-SEAP-26Sfur-3TM-hcvs-KKMP | HCVP-inducible tri-transmembrane SEAP RELEASE construct |
| 179636 | CMVTO-SEAP-26Sfur-B2AD-tevs-KKMP | TEVP-inducible single TM SEAP RELEASE construct |
| 179637 | CMVTO-SEAP-26Sfur-3TM-hcvs-KKMP (GS) | HCVP-inducible tri-transmembrane SEAP RELEASE with GS linkers |
| 179638 | CMVTO-SEAP-26Sfur-B2AD-hcvs-KKMP | HCVP-inducible single TM SEAP RELEASE construct |
| 179639 | CMVTO-SEAP-26Sfur-B2AD-hcvs-KKMP (GS) | HCVP-inducible single TM SEAP RELEASE construct with GS linkers |
| 179640 | CMVTO-GFP-ECL-3TM-tevs-KKMP V3 | TEVP-inducible membrane-bound GFP RELEASE construct |
| 179641 | CMVTO-GFP-ECL-3TM-hcvs-KKMP | HCVP-inducible membrane-bound GFP RELEASE construct |
| 179642 | CMVTO-GFP-26Sfur-3TM-hcvs-KKMP | HCVP-inducible tri-transmembrane GFP RELEASE construct |
| 179643 | CMVTO-SEAP-26sFur-B2AD-hcvs-tevs-KKMP | OR Gate (TEVP/HCVP) SEAP RELEASE construct |
| 179644 | CMVTO-p450-hcvs-B2AD2-26Sfur-SEAP-26sFur-B2AD-tevs-KKMP | AND gate (TEVP/HCVP) SEAP RELEASE construct |
| 179645 | CMVTO-SEAP-26SFur-B2AD-tvmvs-KKMP | TVMVP-inducible single TM SEAP RELEASE construct |
| 179646 | CMVTO-IL12-26Sfur-B2AD-tvmvs-KKMP | TVMVP-inducible IL-12 RELEASE construct |
| 179647 | CMVTO-p450-hcvs-B2AD2-26Sfur-SEAP | HCVP-inducible p450 SEAP RELEASE construct |
| 179648 | CMVTO-SEAP-26Sfur-B2AD-SpyTag-tvmvs-KKMP | TVMVP-inducible single TM SEAP RELEASE construct with SpyTag |
| 179649 | CMVTO-g0 | Non-coding DNA plasmid control |
| 179650 | CMVTO-H2b-Che | mCherry co-transfection marker |
| 179651 | CMVTO-BFP | BFP co-transfection marker |
| 179652 | CMVTO-HRAS-G12V | HRAS active mutant (G12V mutation) |
| 179653 | CMVTO-KRAS | KRAS protein |
| 179654 | CMVTO-KRAS-G12V | KRAS active mutant (G12V mutation) |
| 179655 | CMVTO-KRAS-G12C | KRAS active mutant (G12C mutation) |
| 179656 | CMVTO-KRAS-G12D | KRAS active mutant (G12D mutation) |
| 179657 | CMVTO-TEVP | Tobacco Etch Viral Protease |
| 179658 | CMVTO-HCVP | Hepatitis C Viral Protease |
| 179659 | CMVTO-TVMVP | Tobacco Vein Mottling Viral Protease |
| 179660 | CMVTO-nTVMVP-AP4-tevs-P3-tevs-cTVMVPmut | Cytosolic TEVP-inducible caged-nTVMVP |
| 179661 | CMVTO-nTVMVPmut-tevs-AP4-tevs-P3-cTVMVP | Cytosolic TEVP-inducible caged-cTVMVP |
| 179662 | CMVTO-nTVMVp-AP4-tevs-P3-tevs-cTVMVPmut-CAAX | Membrane-bound (CAAX) TEVP-inducible caged-nTVMVP |
| 179663 | CMVTO-P3-cTVMVP | Uncaged-cTVMVP with P3 leucine zipper |
| 179664 | CMVTO-p450-P3-cTVMVP | ER-associated uncaged-cTVMVP with P3 leucine zipper |
| 179666 | CMVTO-RBDK65E-nTEVp | Ras Binding Domain split-nTEVP |
| 179667 | CMVTO-RBDK65E-cTEVp | Ras Binding Domain split-cTEVP |
| 179668 | CMVTO-Fyn-nTVMVpmut-tevs-AP4-tevs-P3-cTVMVp | Membrane-bound (Fyn) TEVP-inducible caged-cTVMVP |
| 179669 | CMVTO-p450-nTVMVp-AP4 | ER-associated uncaged-nTVMVP with AP4 leucine zipper |
| 179670 | CMVTO-RBDK65E-R89L-nTEVp | Mutant Ras Binding Domain split-nTEVP |
| 179671 | CMVTO-RBDK65E-R89L-cTEVp | Mutant Ras Binding Domain split-cTEVP |
| 179672 | CMVTO-Lyn-nTVMVpmut-tevs-AP4-tevs-P3-cTVMVp | Membrane-bound (Lyn) TEVP-inducible caged-cTVMVP |
| 179673 | CMVTO-p450-nTVMVp-hcvs-AP4 | HCVP-repressible ER-associated uncaged-nTVMVP |
| 179674 | CMVTO-p450-Ns4A-tv-HCVp-P3 | TVMVP-repressible ER-associated HCVP |
| 179675 | CMVTO-p450-FKBP-nTEVP | ER-associated FKBP split nTEVP (Rapalog inducible) |
| 179676 | CMVTO-p450-FRB-cTEVP | ER-associated FRB split-cTEVP (Rapalog inducible) |
| 179677 | CMVTO-p450-hcvs-SpyCatcher | HCVP-cleavable ER-associated SpyCatcher |
| 179704 | CMVTO-mCherry-Kir2.1-HA-tevs-RXR | TEVP-inducible mCherry-conjugated Kir2.1 RELEASE |
| 179705 | CMVTO-GFP-Kir2.1-HA-tevs-RXR-V2 | TEVP-inducible GFP-conjugated Kir2.1 RELEASE |
| 179706 | CMVTO-gfp-Kir2.1-HA-tevs-KKYL | TEVP-inducible GFP-conjugated Kir2.1 RELEASE with KKXX motif |
| 179707 | CMVTO-mCherry-Kir2.1-HA-tvmvs-RXR-V2 | TVMVP-inducible mCherry-conjugated Kir2.1 RELEASE |
| 179908 | CMVTO-SEAP | Secreted Embryonic Alkaline Phosphatase |
| 179912 | CMVTO-HA-GFP | Constitutively secreted GFP |
| 179920 | CMVTO-Furin | Furin protein |
| 180022 | CMVTO-Gbp6-TM-Che | Membrane-bound anti-GFP nanobody conjugated to mCherry |