Comparison of NF-kappaB from the protists Capsaspora owczarzaki and Acanthoeca spectabilis reveals extensive evolutionary diversification of this transcription factor.
Williams LM, Sridhar S, Samaroo J, Peart J, Adindu EK, Addanki A, DiRusso CJ, Aguirre Carrion PJ, Rodriguez-Sastre N, Siggers T, Gilmore TD
Commun Biol. 2021 Dec 16;4(1):1404. doi: 10.1038/s42003-021-02924-2. PubMed Article
Williams LM, Sridhar S, Samaroo J, Peart J, Adindu EK, Addanki A, DiRusso CJ, Aguirre Carrion PJ, Rodriguez-Sastre N, Siggers T, Gilmore TD
Commun Biol. 2021 Dec 16;4(1):1404. doi: 10.1038/s42003-021-02924-2. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 180197 | pcDNA-FLAG-Co-NF-kB | Expresses FLAG-tagged Capsaspora NF-kB protein in mammalian cells |
| 180199 | pcDNA-FLAG-Co-NF-kB-Cterm | Expresses FLAG-tagged Capsaspora NF-kB C-terminal sequences in mammalian cells |
| 180200 | pcDNA-FLAG-As-NF-kB1 | Expresses FLAG-tagged Acanthoeca spectabilis NF-kB1 protein in mammalian cells |
| 180202 | pcDNA-FLAG-As-NF-kB3 | Expresses FLAG-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cells |
| 180203 | pcDNA-MYC-As-NF-kB1 | Expresses MYC-tagged Acanthoeca spectabilis NF-kB1 protein in mammalian cells |
| 180204 | pcDNA-MYC-As-NF-kB2 | Expresses MYC-tagged Acanthoeca spectabilis NF-kB2 protein in mammalian cells |
| 180206 | pGB-Co-NF-kB | Expression GAL4-fusion of Co-NF-kB in yeast |
| 180207 | pGB-Co-RHD | Expresses GAL4 fusion of Capsaspora NF-kB Rel Homology Domain sequences in yeast |
| 180208 | pGB-Co-Cterm | Expresses GAL4 fusion of Capsaspora NF-kB C-terminal sequences in yeast |
| 180209 | pGB-As-NF-kB1 | Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB1 protein in yeast |
| 180210 | pGB-As-NF-kB2 | Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB2 protein in yeast |
| 180211 | pGB-As-NF-kB3 | Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB3 protein in yeast |